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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Hamster Interleukin 1β ELISA kit

E18I0010 Hamster Interleukin 1β ELISA kit

Hamster Interleukin 1β ELISA kit can be used to identify samples from the Hamster species. Interleukin 1β  can also be called Catabolin, H1, IL-1β, Hematopoietin 1, IFN beta inducing factor, IL 1, IL 1 beta, IL 1B, IL1B, IL1F2, Interleukin 1 beta, Interleukin 1 beta precursor, LAF, OAF, Osteoclast activating factor, Preinterleukin beta, Pro interleukin 1 beta, IInterleukin-1 beta.

Specifications of Hamster Interleukin 1β ELISA kit

Product Information

Cat. No.

E18I0010

Product Name

Hamster Interleukin 1β ELISA kit

Species

Hamster

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL 1β ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 1β antibody and an IL 1β-HRP conjugate. The assay sample and buffer are incubated together with IL 1β-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 1β concentration since IL 1β from samples and IL 1β-HRP conjugate compete for the anti-IL 1β antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 1β from the sample, fewer sites are left to bind IL 1β-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 1β concentration in each sample is interpolated from this standard curve.


Quality Control on Hamster Interleukin 1β ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL 1β and analogues was observed.


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Summary of the Assay Procedure for Hamster Interleukin 1β ELISA kit

Summary of the Assay Procedure for Hamster Interleukin 1β ELISA kit

Citations of Hamster Interleukin 1β ELISA kit

E18I0010 has been referenced in the below publications:

Effects of fish oil and spirulina on oxidative stress and inflammation in hypercholesterolemic hamsters.

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