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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Gastric Inhibitory Polypeptide ELISA kit (E01G0177)

E01G0177 Human Gastric Inhibitory Polypeptide ELISA kit

The Human Gastric Inhibitory Polypeptide ELISA kit can be used to identify samples from the human species. Human Gastric Inhibitory Polypeptide ELISA kit can also be called Gastric Inhibitory polypeptide, Gastric inhibitory polypeptide precursor, Glucose dependent insulinotropic polypeptide, Gastric Inhibitory Peptide, Glucose-dependent insulinotropic polypeptide, Incretin hormone.

Products

Specifications of Human Gastric Inhibitory Polypeptide ELISA kit

Product Information

Cat. No.

E01G0177

Product Name

Human Gastric Inhibitory Polypeptide ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

5.0 pg/mL

1 vial

STANDARD C (0.5mL)

10 pg/mL

1 vial

STANDARD D (0.5mL)

25 pg/mL

1 vial

STANDARD E (0.5mL)

50 pg/mL

1 vial

STANDARD F (0.5mL)

100 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GIP ELISA kit uses an anti-GIP antibody and an GIP-HRP conjugate in a competitive enzyme immunoassay method. GIP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GIP antibody binding site between GIP from samples and GIP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GIP. Since the number of sites is limited, as more sites are occupied by GIP from the sample, fewer sites are left to bind GIP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GIP concentration in each sample is interpolated from this standard curve.


Quality Control on Human Gastric Inhibitory Polypeptide ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GIP and analogues was observed.


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Summary of the Assay Procedure for Human Gastric Inhibitory Polypeptide ELISA kit

Summary of the Assay Procedure for Human Gastric Inhibitory Polypeptide ELISA kit

Citations of Human Gastric Inhibitory Polypeptide ELISA kit

E01G0177 has been referenced in the below publications:

Effect of oral glutamine on the secretion of plasma GLP-1 in patients with type 2 diabetes.

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