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BlueGene Biotech Human Gastrointestinalcancer Marker CA199 ELISA kit

E01G0307 Human Gastrointestinalcancer Marker CA199 ELISA kit

The Human Gastrointestinalcancer Marker CA199 ELISA kit can be used to identify samples from the human species. Gastrointestinalcancer Marker CA199 can also be called CA199, CA19-9, sialyl LeA, SLeA, cancer antigen 19-9, Gastrointestinal cancer Marker CA199.

Specifications of Human Gastrointestinalcancer Marker CA199 ELISA kit

Product Information

Cat. No.

E01G0307

Product Name

Human Gastrointestinalcancer Marker CA199 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 U/mL

Sensitivity

0.1 U/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 U/mL

1 vial

STANDARD B (0.5mL)

1.0 U/mL

1 vial

STANDARD C (0.5mL)

2.5 U/mL

1 vial

STANDARD D (0.5mL)

5.0 U/mL

1 vial

STANDARD E (0.5mL)

10 U/mL

1 vial

STANDARD F (0.5mL)

25 U/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Gastrointestinalcancer Marker CA199 ELISA kit uses an anti-Gastrointestinalcancer Marker CA199 antibody and an Gastrointestinalcancer Marker CA199-HRP conjugate in a competitive enzyme immunoassay method. Gastrointestinalcancer Marker CA199-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Gastrointestinalcancer Marker CA199 antibody binding site between Gastrointestinalcancer Marker CA199 from samples and Gastrointestinalcancer Marker CA199-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Gastrointestinalcancer Marker CA199. Since the number of sites is limited, as more sites are occupied by Gastrointestinalcancer Marker CA199 from the sample, fewer sites are left to bind Gastrointestinalcancer Marker CA199-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Gastrointestinalcancer Marker CA199 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Gastrointestinalcancer Marker CA199 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Gastrointestinalcancer Marker CA199 and   analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Gastrointestinalcancer Marker CA199 ELISA kit

Summary of the Assay Procedure for Human Gastrointestinalcancer Marker CA199 ELISA kit

Citations of Human Gastrointestinalcancer Marker CA199 ELISA kit

E01G0307 has been referenced in the below publications:

The study on the detection of AFP, AFU, CA199 and GPC3 separately or combined in the diagnosis of primary hepatic cancer


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