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BlueGene Biotech Human Immunoglobulin M ELISA kit (E01I0038)

E01I0038 Human Immunoglobulin M ELISA kit

The Human Immunoglobulin M ELISA kit can be used to identify samples from the human species. Immunoglobulin M can also be called IgM, IGHM, Immunoglobulin Heavy Constant Mu, Ig mu chain C region.

Products

Specifications of Human Immunoglobulin M ELISA kit

Product Information

Cat. No.

E01I0038

Product Name

Human Immunoglobulin M ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IgM ELISA kit uses an anti-IgM antibody and an IgM-HRP conjugate in a competitive enzyme immunoassay method. IgM-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IgM antibody binding site between IgM from samples and IgM-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IgM. Since the number of sites is limited, as more sites are occupied by IgM from the sample, fewer sites are left to bind IgM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgM concentration in each sample is interpolated from this standard curve.


Quality Control on Human Immunoglobulin M ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IgM and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Immunoglobulin M ELISA kit

Summary of the Assay Procedure for Human Immunoglobulin M ELISA kit

Citations of Human Immunoglobulin M ELISA kit

E01I0038 has been referenced in the below publications:

Protective Effect of Bisoprolol Preconditioning on Myocardial Ischemia Reperfusion Injury in Rats by the Medium of PI3K/AKT/GS3K-b.

The serum levels of IL-17 and IL-23 in children with Mycoplasma pneumoniae pneumonia.

The Clinical research of Ma Xing Gan Shi treating Pathogenic heat which Basing on the mode of Sequential administration.

hs-CRP与免疫功能检测在肺炎支原体肺炎诊治中的意义探讨。

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