E01C0613 Human Neopterin ELISA kit
The Human Neopterin ELISA kit can be used to identify samples from the human species. Neopterin can also be called 2-Amino-6-(1,2,3-trihydroxypropyl)pteridin-4(1H)-one.
Human Neopterin ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
NP ELISA kit uses an anti-NP antibody and an NP-HRP conjugate in a competitive enzyme immunoassay method. NP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NP antibody binding site between NP from samples and NP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NP. Since the number of sites is limited, as more sites are occupied by NP from the sample, fewer sites are left to bind NP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NP concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between NP and analogues was observed.
E01N0603 has been referenced in the below publications：
Inflammatory Bowel Disease-Associated Changes in the Gut:Focus on Kazan Patients.
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