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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Placenta Growth Factor ELISA kit

E01P0018 Human Placenta Growth Factor ELISA kit

The Human Placenta Growth Factor ELISA kit can be used to identify samples from the human species. Placenta Growth Factor can also be called PlGF2, PGF, PGFL, PLGF, PIGF, Placental Growth Factor-Like, Vascular Endothelial Growth Factor-Related Protein, D12S1900, PlGF-2, SHGC-10760, Placental growth factor.

Specifications of Human Placenta Growth Factor ELISA kit

Product Information

Cat. No.

E01P0018

Product Name

Human Placenta Growth Factor ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PLGF ELISA kit uses an anti-PLGF antibody and a PLGF-HRP conjugate in a competitive enzyme immunoassay method. PLGF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PLGF antibody binding site between PLGF from samples and PLGF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PLGF. Since the number of sites is limited, as more sites are occupied by PLGF from the sample, fewer sites are left to bind PLGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLGF concentration in each sample is interpolated from this standard curve.


Quality Control on Human Placenta Growth Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PLGF and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Placenta Growth Factor ELISA kit

Summary of the Assay Procedure for Human Placenta Growth Factor ELISA kit

Citations of Human Placenta Growth Factor ELISA kit

E01P0018 has been referenced in the below publications:

Pilot study of bevacizumab in combination with docetaxel and cyclophosphamide as adjuvant treatment for patients with early stage HER-2 negative breast cancer, including analysis of candidate circulating markers of cardiac toxicity: ICORG 08-10 trial.

What is the impact of intravitreal injection of conbercept on neovascular glaucoma patients: a prospective, interventional case series study.

孕中晚期PLGF、Sflt-1、sEng的MoM参考值区间研究。

The role and mechanism of asymmetric dimethylarginine in fetal growth restriction via interference with endothelial function and angiogenesis.

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