En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Anti Double Stranded DNA ELISA kit

  • Epigenetics and Nuclear Signaling

E03D0004 Mouse Anti Double Stranded DNA ELISA kit

Mouse Anti Double Stranded DNA ELISA kit is suitable for detecting samples from mouse species. Anti Double Stranded DNA can also be called dsDNA-Ab, anti dsDNA.

Specifications of Mouse Anti Double Stranded DNA ELISA kit

Product Information

Cat. No.

E03D0004

Product Name

Mouse Anti Double Stranded DNA ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

anti dsDNA ELISA kit applies the competitive enzyme immunoassay technique utilizing a dsDNA antigen and an anti dsDNA-HRP conjugate. The assay sample and buffer are incubated together with anti dsDNA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the anti dsDNA concentration since anti dsDNA from samples and anti dsDNA-HRP conjugate compete for the dsDNA antigen binding site. Since the number of sites is limited, as more sites are occupied by anti dsDNA from the sample, fewer sites are left to bind anti dsDNA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The anti dsDNA concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Anti Double Stranded DNA ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

98-104%

Linearity

Diluent Ratio

Range %

1:2

96-103

1:4

95-107

1:8

91-102

Specificity/Cross-reactivity

No significant cross-reactivity or interference between anti dsDNA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Anti Double Stranded DNA ELISA kit

Summary of the Assay Procedure for Mouse Anti Double Stranded DNA ELISA kit

Citations of Mouse Anti Double Stranded DNA ELISA kit

E03D0004 has been referenced in the below publications:

The effect of langchuangqing granule on anti-ds-DNA antibody,IgG and IgA level in MRL/lpr lupus mice.

Toll-like receptor 2-modulating pectin-polymers in alginate-based microcapsules attenuate immune responses and support islet-xenograft survival.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.