E03D0224 Mouse D Dimer ELISA kit
The Mouse D Dimer ELISA kit can be used to identify samples from the mouse species. D Dimer can also be called D2D, D 2 Dimer, D-Dimer.
Mouse D Dimer ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
D2D ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-D2D antibody and an D2D-HRP conjugate. The assay sample and buffer are incubated together with D2D-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the D2D concentration since D2D from samples and D2D-HRP conjugate compete for the anti-D2D antibody binding site. Since the number of sites is limited, as more sites are occupied by D2D from the sample, fewer sites are left to bind D2D-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The D2D concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between D2D and analogues was observed.
E03D0224 has been referenced in the below publications：
Molecular Mimicry between Dengue Virus and Coagulation Factors Induces Antibodies To Inhibit Thrombin Activity and Enhance Fibrinolysis.
S100ß, Matrix Metalloproteinase-9, D-dimer, and Heat Shock Protein 70 Are Serologic Biomarkers of Acute Cerebral Infarction in a Mouse Model of Transient MCA Occlusion.
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