E03G0074 Mouse Gamma Aminobutyric Acid ELISA kit
The Mouse Gamma Aminobutyric Acid ELISA kit can be used to identify samples from the mouse species. Gamma Aminobutyric Acid can also be called GABA, γ-Aminobutanoic acid, 4-Aminobutyric acid, 3-Carboxypropylamine, Piperidic acid, Piperidinic acid.
Product Information | |
Cat. No. | E03G0074 |
Product Name | Mouse Gamma Aminobutyric Acid ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GABA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GABA antibody and an GABA-HRP conjugate. The assay sample and buffer are incubated together with GABA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GABA concentration since GABA from samples and GABA-HRP conjugate compete for the anti-GABA antibody binding site. Since the number of sites is limited, as more sites are occupied by GABA from the sample, fewer sites are left to bind GABA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GABA concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GABA and analogues was observed. | |
E03G0074 has been referenced in the below publications：
Effects of chronic noise exposure on the microbiome-gut-brain axis in senescence-accelerated prone mice:implications for Alzheimer’s disease.
Environmental noise stress disturbs commensal microbiota homeostasis and induces oxi-inflammmation and AD-like neuropathology through epithelial barrier disruption in the EOAD mouse model.
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