E03O0023 Mouse Oxytocin ELISA kit
The Mouse Oxytocin ELISA kit can be used to identify samples from the mouse species. Oxytocin can also be called Pitocin, Syntocinon, OT.
Mouse Oxytocin ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
|0 pg/ml||1 vial|
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
OT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-OT antibody and an OT-HRP conjugate. The assay sample and buffer are incubated together with OT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OT concentration since OT from samples and OT-HRP conjugate compete for the anti-OT antibody binding site. Since the number of sites is limited, as more sites are occupied by OT from the sample, fewer sites are left to bind OT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OT concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between OT and analogues was observed.
E03O0023 has been referenced in the below publications：
Studies of pharmacodynamic material basis of Eucommia ulmoides Oliv.
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