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  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Stromal Cell Derived Fctor 1Alpha ELISA kit (E03S0006)

E03S0006 Mouse Stromal Cell Derived Fctor 1Alpha ELISA kit

The Mouse Stromal Cell Derived Fctor 1Alpha ELISA kit can be used to identify samples from the mouse species. Stromal Cell Derived Fctor 1Alpha can also be called CXCL12, SDF-1, and SDF1Alpha.

Products

Specifications of Mouse Stromal Cell Derived Fctor 1Alpha ELISA kit

Product Information

Cat. No.

E03S0006

Product Name

Mouse Stromal Cell Derived Fctor 1Alpha ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

0.5-10ng/ml

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml1 vial

STANDARD B (0.5mL)

0.5 ng/ml

1 vial

STANDARD C (0.5mL)

1.0 ng/ml

1 vial

STANDARD D (0.5mL)

2.5 ng/ml

1 vial

STANDARD E (0.5mL)

5.0ng/ml

1 vial

STANDARD F (0.5mL)

10 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SDF1Alpha  ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SDF1Alpha  antibody and a SDF1Alpha -HRP conjugate. The assay sample and buffer are incubated together with SDF1Alpha -HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SDF1Alpha  concentration since SDF1Alpha  from samples and SDF1Alpha -HRP conjugate compete for the anti-SDF1Alpha  antibody binding site. Since the number of sites is limited, as more sites are occupied by SDF1Alpha  from the sample, fewer sites are left to bind SDF1Alpha -HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDF1Alpha  concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Stromal Cell Derived Fctor 1Alpha ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SDF1Alpha  and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Stromal Cell Derived Fctor 1Alpha ELISA kit

Summary of the Assay Procedure for Mouse Stromal Cell Derived Fctor 1Alpha ELISA kit

Citations of Mouse Stromal Cell Derived Fctor 1Alpha ELISA kit

E03S0006 has been referenced in the below publications:

Reverse-D-4F Increases the Number of Endothelial Progenitor Cells and Improves Endothelial Progenitor Cell Dysfunctions in High Fat Diet Mice.


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