E03A0056 Mouse Antioncogene P62 Protein ELISA kit
Mouse Antioncogene p62 protein ELISA kit is suitable for the detection of samples from mouse species. Antioncogene p62 protein can also be called p62.
Specifications of Mouse Antioncogene P62 Protein ELISA kit
Product Information | |
Cat. No. | E03A0056 |
Product Name | Mouse Antioncogene p62 protein ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
p62 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-p62 antibody and an p62-HRP conjugate. The assay sample and buffer are incubated together with p62-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the p62 concentration since p62 from samples and p62-HRP conjugate compete for the anti-p62 antibody binding site. Since the number of sites is limited, as more sites are occupied by p62 from the sample, fewer sites are left to bind p62-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The p62 concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Antioncogene P62 Protein ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 92-108% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-114 | |
1:4 | 92-109 | |
1:8 | 95-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between p62 and analogues was observed. | |
Summary of the Assay Procedure for Mouse Antioncogene P62 Protein ELISA kit
Citations of Mouse Antioncogene P62 Protein ELISA kit
E03A0056 has been referenced in the below publications:
The suppressed autophagy induced by carbon disulfide could be rescued by N-carbamoyl glutamate during the window of embryo implantation in mice.
