En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Antioncogene P62 Protein ELISA kit (E03A0056)

E03A0056 Mouse Antioncogene P62 Protein ELISA kit

Mouse Antioncogene p62 protein ELISA kit is suitable for the detection of samples from mouse species. Antioncogene p62 protein can also be called p62.

Products

Specifications of Mouse Antioncogene P62 Protein ELISA kit

Product Information

Cat. No.

E03A0056

Product Name

Mouse Antioncogene p62 protein ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

p62 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-p62 antibody and an p62-HRP conjugate. The assay sample and buffer are incubated together with p62-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the p62 concentration since p62 from samples and p62-HRP conjugate compete for the anti-p62 antibody binding site. Since the number of sites is limited, as more sites are occupied by p62 from the sample, fewer sites are left to bind p62-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The p62 concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse Antioncogene P62 Protein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

92-108%

Linearity

Diluent Ratio

Range %

1:2

96-114

1:4

92-109

1:8

95-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between p62 and analogues was observed.


Summary of the Assay Procedure for Mouse Antioncogene P62 Protein ELISA kit

Summary of the Assay Procedure for Mouse Antioncogene P62 Protein ELISA kit

Citations of Mouse Antioncogene P62 Protein ELISA kit

E03A0056 has been referenced in the below publications:

The suppressed autophagy induced by carbon disulfide could be rescued by N-carbamoyl glutamate during the window of embryo implantation in mice.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.