E03A0449 Mouse Anti Histone Antibody ELISA kit
Mouse Anti Histone Antibody ELISA kit is suitable for detecting samples from mouse species. Anti Histone Antibody can also be called HIS-Ab, AHA.
Mouse Anti Histone Antibody ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
AHA ELISA kit applies the competitive enzyme immunoassay technique utilizing a Histone antigen and an AHA-HRP conjugate. The assay sample and buffer are incubated together with AHA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AHA concentration since AHA from samples and AHA-HRP conjugate compete for the Histone antigen binding site. Since the number of sites is limited, as more sites are occupied by AHA from the sample, fewer sites are left to bind AHA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AHA concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between AHA and analogues was observed.
E03A0449 has been referenced in the below publications:
Transplanted Human Umbilical Cord Mesenchymal Stem Cells Facilitate Lesion Repair in B6.Fas Mice.
Relationship between p16 protein expression and prognosis in patients with gastric adenocarcinoma treated with XELOX chemotherapy.
Related Bluegene Biotech Products