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  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Anti Histone Antibody ELISA kit (E03A0449)

E03A0449 Mouse Anti Histone Antibody ELISA kit

Mouse Anti Histone Antibody ELISA kit is suitable for detecting samples from mouse species. Anti Histone Antibody can also be called HIS-Ab, AHA.

Products

Specifications of Mouse Anti Histone Antibody ELISA kit

Product Information

Cat. No.

E03A0449

Product Name

Mouse Anti Histone Antibody ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AHA ELISA kit applies the competitive enzyme immunoassay technique utilizing a Histone antigen and an AHA-HRP conjugate. The assay sample and buffer are incubated together with AHA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AHA concentration since AHA from samples and AHA-HRP conjugate compete for the Histone antigen binding site. Since the number of sites is limited, as more sites are occupied by AHA from the sample, fewer sites are left to bind AHA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AHA concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Anti Histone Antibody ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

98-104%

Linearity

Diluent Ratio

Range %

1:2

96-103

1:4

95-107

1:8

91-102

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AHA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Anti Histone Antibody ELISA kit

Summary of the Assay Procedure for Mouse Anti Histone Antibody ELISA kit

Citations of Mouse Anti Histone Antibody ELISA kit

E03A0449 has been referenced in the below publications:

Transplanted Human Umbilical Cord Mesenchymal Stem Cells Facilitate Lesion Repair in B6.Fas Mice.

Relationship between p16 protein expression and prognosis in patients with gastric adenocarcinoma treated with XELOX chemotherapy.

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