E03A0040 Mouse Anti Nuclear Antibody ELISA kit
Mouse Anti nuclear Antibody ELISA kit is suitable for detecting samples from mouse species. Anti nuclear Antibody can also be called ANA, antinuclear factor, ANF.
Specifications of Mouse Anti Nuclear Antibody ELISA kit
Product Information | |
Cat. No. | E03A0040 |
Product Name | Mouse Anti nuclear Antibody ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ANA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ANA antibody and an ANA-HRP conjugate. The assay sample and buffer are incubated together with ANA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANA concentration since ANA from samples and ANA-HRP conjugate compete for the anti-ANA antibody binding site. Since the number of sites is limited, as more sites are occupied by ANA from the sample, fewer sites are left to bind ANA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANA concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Anti Nuclear Antibody ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 98-104% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-103 | |
1:4 | 95-107 | |
1:8 | 91-102 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ANA and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Anti Nuclear Antibody ELISA kit
Citations of Mouse Anti Nuclear Antibody ELISA kit
E03A0040 has been referenced in the below publications:
Transplanted Human Umbilical Cord Mesenchymal Stem Cells Facilitate Lesion Repair in B6.Fas Mice.
Relationship between p16 protein expression and prognosis in patients with gastric adenocarcinoma treated with XELOX chemotherapy.
