E03B0697 Mouse Blood Urea Nitrogen ELISA kit
The Mouse Blood Urea Nitrogen ELISA kit can be used to identify samples from the mouse species. Blood Urea Nitrogen can also be called BUN.
Mouse Blood Urea Nitrogen ELISA kitv
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
BUN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BUN antibody and an BUN-HRP conjugate. The assay sample and buffer are incubated together with BUN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BUN concentration since BUN from samples and BUN-HRP conjugate compete for the anti-BUN antibody binding site. Since the number of sites is limited, as more sites are occupied by BUN from the sample, fewer sites are left to bind BUN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BUN concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between BUN and analogues was observed.
E03B0697 has been referenced in the below publications：
Anti—Fatigue Activity of Flavonoids from Herbahouttuynia.
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