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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Insulin Receptor Substrate 1 ELISA kit

E03I0403 Mouse Insulin Receptor Substrate 1 ELISA kit

The Mouse Insulin Receptor Substrate 1 ELISA kit can be used to identify samples from the mouse species. Insulin Receptor Substrate 1 can also be called IRS-1, IRS1, HIRS-1, insulin receptor substrate 1, IRS 1.

Specifications of Mouse Insulin Receptor Substrate 1 ELISA kit

Product Information

Cat. No.

E03I0403

Product Name

Mouse Insulin Receptor Substrate 1 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

1.0-25ng/ml

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml1 vial

STANDARD B (0.5mL)

1.0 ng/ml

1 vial

STANDARD C (0.5mL)

2.5 ng/ml

1 vial

STANDARD D (0.5mL)

5.0 ng/ml

1 vial

STANDARD E (0.5mL)

10 ng/ml

1 vial

STANDARD F (0.5mL)

25 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IRS 1 ELISA kit uses an anti-IRS 1 antibody and an IRS 1-HRP conjugate in a competitive enzyme immunoassay method. IRS 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IRS 1 antibody binding site between IRS 1 from samples and IRS 1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IRS 1. Since the number of sites is limited, as more sites are occupied by IRS 1 from the sample, fewer sites are left to bind IRS 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IRS 1 concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse Insulin Receptor Substrate 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IRS 1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Insulin Receptor Substrate 1 ELISA kit

Summary of the Assay Procedure for Mouse Insulin Receptor Substrate 1 ELISA kit

Citations of Mouse Insulin Receptor Substrate 1 ELISA kit

E03I0403 has been referenced in the below publications:

Effect of Different Factors of Moxibustion on P13K/AKT Signal Pathway and Cortical 6-amyioid Protein Precipitation in Brain Tissue of Alzheimer Mice.

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