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BlueGene Biotech Mouse Peroxisome Proliferators Activated Receptor γ ELISA kit (E03P0045)

E03P0045 Mouse Peroxisome Proliferators Activated Receptor γ ELISA kit

The Mouse Peroxisome Proliferators Activated Receptor γ ELISA kit can be used to identify samples from the mouse species. Peroxisome Proliferators Activated Receptor γ can also be called PPAR-G, PPARG1, PPARG2, NR1C3, Glitazone Receptor, Nuclear Receptor Subfamily 1 Group C Member 3, PPARγ.

Products

Specifications of Mouse Peroxisome Proliferators Activated Receptor γ ELISA kit

Product Information

Cat. No.

E03P0045

Product Name

Mouse Peroxisome Proliferators Activated Receptor γ ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

1.0-25ng/ml

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml1 vial

STANDARD B (0.5mL)

1.0 ng/ml

1 vial

STANDARD C (0.5mL)

2.5 ng/ml

1 vial

STANDARD D (0.5mL)

5.0 ng/ml

1 vial

STANDARD E (0.5mL)

10 ng/ml

1 vial

STANDARD F (0.5mL)

25 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PPARγ ELISA kit uses an anti-PPARγ antibody and an PPARγ-HRP conjugate in a competitive enzyme immunoassay method. PPARγ-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PPARγ antibody binding site between PPARγ from samples and PPARγ-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PPARγ. Since the number of sites is limited, as more sites are occupied by PPARγ from the sample, fewer sites are left to bind PPARγ-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PPARγ concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse Peroxisome Proliferators Activated Receptor γ ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PPARγ and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Peroxisome Proliferators Activated Receptor γ ELISA kit

Summary of the Assay Procedure for Mouse Peroxisome Proliferators Activated Receptor γ ELISA kit

Citations of Mouse Peroxisome Proliferators Activated Receptor γ ELISA kit

E03P0045 has been referenced in the below publications:

Triglyceride with medium-chain fatty acids increases the activity and expression of hormone-sensitive lipase in white adipose tissue of C57BL/6J mice.

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