E03P0668 Mouse Protein Kinase C ELISA kit
The Mouse Protein Kinase C ELISA kit can be used to identify samples from the mouse species. Protein Kinase C can also be called PKC．
Mouse Protein Kinase C ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
|0 ng/ml||1 vial|
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
PKC ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PKC antibody and a PKC-HRP conjugate. The assay sample and buffer are incubated together with PKC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKC concentration since PKC from samples and PKC-HRP conjugate compete for the anti-PKC antibody binding site. Since the number of sites is limited, as more sites are occupied by PKC from the sample, fewer sites are left to bind PKC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKC concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between PKC and analogues was observed.
E03P0668 has been referenced in the below publications：
Quercetin and lithium chloride potentiate the protective effects of carvedilol against renal ischemia-reperfusion injury in high-fructose, high-fat diet-fed Swiss albino mice independent of renal lipid signaling.
Carvedilol protects against hepatic ischemia/reperfusion injury in high-fructose/high-fat diet-fed mice: Role of G protein-coupled receptor kinase 2 and 5.
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