E07I0038 Porcine Immunoglobulin M ELISA kit
The Porcine Immunoglobulin M ELISA kit can be used to identify samples from the porcine species. Immunoglobulin M can also be called IGHM, Immunoglobulin Heavy Constant Mu, Ig mu chain C region.
Specifications of Porcine Immunoglobulin M ELISA kit
Product Information | |
Cat. No. | E07I0038 |
Product Name | Porcine Immunoglobulin M ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 mg/ml |
Sensitivity | 0.1 mg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mg/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 mg/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 mg/mL | 1 vial |
STANDARD D (0.5mL) | 10 mg/mL | 1 vial |
STANDARD E (0.5mL) | 25 mg/mL | 1 vial |
STANDARD F (0.5mL) | 50 mg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IgM ELISA kit uses an anti-IgM antibody and an IgM-HRP conjugate in a competitive enzyme immunoassay method. IgM-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IgM antibody binding site between IgM from samples and IgM-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IgM. Since the number of sites is limited, as more sites are occupied by IgM from the sample, fewer sites are left to bind IgM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgM concentration in each sample is interpolated from this standard curve. |
Quality Control on Porcine Immunoglobulin M ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IgM and analogues was observed. | |
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Summary of the Assay Procedure for Porcine Immunoglobulin M ELISA kit
Citations of Porcine Immunoglobulin M ELISA kit
E07I0038 has been referenced in the below publications：
Effects of feeding an herbal preparation to sows on immunological performance of offspring.
