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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Immunoglobulin M ELISA kit (E07I0038)

E07I0038 Porcine Immunoglobulin M ELISA kit

The Porcine Immunoglobulin M ELISA kit can be used to identify samples from the porcine species. Immunoglobulin M can also be called IGHM, Immunoglobulin Heavy Constant Mu, Ig mu chain C region.

Products

Specifications of Porcine Immunoglobulin M ELISA kit

Product Information

Cat. No.

E07I0038

Product Name

Porcine Immunoglobulin M ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 mg/ml

Sensitivity

0.1 mg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 mg/mL

1 vial

STANDARD B (0.5mL)

2.5 mg/mL

1 vial

STANDARD C (0.5mL)

5.0 mg/mL

1 vial

STANDARD D (0.5mL)

10 mg/mL

1 vial

STANDARD E (0.5mL)

25 mg/mL

1 vial

STANDARD F (0.5mL)

50 mg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IgM ELISA kit uses an anti-IgM antibody and an IgM-HRP conjugate in a competitive enzyme immunoassay method. IgM-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IgM antibody binding site between IgM from samples and IgM-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IgM. Since the number of sites is limited, as more sites are occupied by IgM from the sample, fewer sites are left to bind IgM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgM concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Immunoglobulin M ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IgM and analogues was observed.


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Summary of the Assay Procedure for Porcine Immunoglobulin M ELISA kit

Summary of the Assay Procedure for Porcine Immunoglobulin M ELISA kit

Citations of Porcine Immunoglobulin M ELISA kit

E07I0038 has been referenced in the below publications:

Effects of feeding an herbal preparation to sows on immunological performance of offspring.

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