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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat alpha-actin ELISA kit

E02A2152 Rat alpha-actin ELISA kit

The Rat alpha-actin ELISA kit can be used to identify samples from the rat species. alpha-actin can also be called actin-binding protein;ACTA1, ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3, Actin, alpha 1, SHPM, actin, alpha 1, skeletal muscle, actin alpha 1, skeletal muscle.

Specifications of Rat alpha-actin ELISA kit

Product Information

Cat. No.

E02A2152

Product Name

Rat alpha-actin ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

aACT ELISA kit uses an anti-aACT antibody and an aACT-HRP conjugate in a competitive enzyme immunoassay method. aACT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-aACT antibody binding site between aACT from samples and aACT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of aACT. Since the number of sites is limited, as more sites are occupied by aACT from the sample, fewer sites are left to bind aACT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The aACT concentration in each sample is interpolated from this standard curve.


Quality Control on Rat alpha-actin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between aACT and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat alpha-actin ELISA kit

Summary of the Assay Procedure for Rat alpha-actin ELISA kit

Citations of Rat alpha-actin ELISA kit

E02A2152 has been referenced in the below publications:

细胞代数与5-氮胞苷浓度对体外诱导骨髓间充质干细胞向心肌样细胞定向分化的影响。


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