E02E0134 Rat Endotoxin ELISA kit
The Rat Endotoxin ELISA kit can be used to identify samples from the rat species. Endotoxin can also be called Lipopolysaccharides, LPS, EDT.
Rat Endotoxin ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
EDT ELISA kit uses an anti-EDT antibody and an EDT-HRP conjugate in a competitive enzyme immunoassay method. EDT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-EDT antibody binding site between EDT from samples and EDT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of EDT. Since the number of sites is limited, as more sites are occupied by EDT from the sample, fewer sites are left to bind EDT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EDT concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between EDT and analogues was observed.
E02E0134 has been referenced in the below publications：
Protective effects and mechanisms of betaine on liver cirrhosis induced by multiple pathogenic factors in rats.
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