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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Myelin Basic Protein ELISA kit

E02M0034 Rat Myelin Basic Protein ELISA kit

The Rat Myelin Basic Protein ELISA kit can be used to identify samples from the rat species. Myelin Basic Protein can also be called Myelin Basic Protein, GDB, Golli MBP, Hemopoietic MBP, HMBPR, HUGO, MLD, Myelin A1 Protein, Myelin Deficient, Myelin Membrane Encephalitogenic Protein, SHI, Shiverer, SP, MBP.

Specifications of Rat Myelin Basic Protein ELISA kit

Product Information

Cat. No.

E02M0034

Product Name

Rat Myelin Basic Protein ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

25-500 ng/mL

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MBP ELISA kit uses an anti-MBP antibody and an MBP-HRP conjugate in a competitive enzyme immunoassay method. MBP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MBP antibody binding site between MBP from samples and MBP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MBP. Since the number of sites is limited, as more sites are occupied by MBP from the sample, fewer sites are left to bind MBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MBP concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Myelin Basic Protein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MBP and analogues was observed.


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Summary of the Assay Procedure for Rat Myelin Basic Protein ELISA kit

Summary of the Assay Procedure for Rat Myelin Basic Protein ELISA kit

Citations of Rat Myelin Basic Protein ELISA kit

E02M0034 has been referenced in the below publications:

Mechanism of delayed encephalopathy after acute carbon monoxide intoxication in rats and intervention of dexamethasone.


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