E02M0032 Rat Myeloperoxidase ELISA kit
The Rat Myeloperoxidase ELISA kit can be used to identify samples from the rat species. Myeloperoxidase can also be called 89 kDa myeloperoxidase, 84 kDa yeloperoxidase, Myeloperoxidase heavy chain, PERM, MPO.
Specifications of Rat Myeloperoxidase ELISA kit
Product Information | |
Cat. No. | E02M0032 |
Product Name | Rat Myeloperoxidase ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MPO ELISA kit uses an anti-MPO antibody and an MPO-HRP conjugate in a competitive enzyme immunoassay method. MPO-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MPO antibody binding site between MPO from samples and MPO-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MPO. Since the number of sites is limited, as more sites are occupied by MPO from the sample, fewer sites are left to bind MPO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MPO concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Myeloperoxidase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MPO and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Myeloperoxidase ELISA kit
Citations of Rat Myeloperoxidase ELISA kit
E02M0032 has been referenced in the below publications：
Therapeutic Effect of Intravenous Infusion of Perfluorocarbon Emulsion on LPS-Induced Acute Lung Injury in Rats.
The Role of Resolvin D1 on acute reflux esophagitis with DHA intervention in Rats.
Phosphocreatine enhances the protective effects of ischemic postconditioning on myocardial ischaemia-reperfusion injury in rats.
Role of autophagy in regulating inflammation in rat models with lipopolysaccharide-induced acute lung injury.
