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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Rheumatoid Factor ELISA kit (E02R0004)

E02R0004 Rat Rheumatoid Factor ELISA kit

The Rat Rheumatoid Factor ELISA kit can be used to identify samples from the rat species. Rheumatoid Factor can also be called RF.

Products

Specifications of Rat Rheumatoid Factor ELISA kit

Product Information

Cat. No.

E02R0004

Product Name

Rat Rheumatoid Factor ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

5-100 IU/ml

Sensitivity

1.0 IU/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 IU/mL

1 vial

STANDARD B (0.5mL)

50 IU/mL

1 vial

STANDARD C (0.5mL)

10 IU/mL

1 vial

STANDARD D (0.5mL)

25 IU/mL

1 vial

STANDARD E (0.5mL)

50 IU/mL

1 vial

STANDARD F (0.5mL)

100 IU/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

RF ELISA kit uses an anti-IRF antibody and an IRF-HRP conjugate in a competitive enzyme immunoassay method. IRF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IRF antibody binding site between IRF from samples and IRF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IRF. Since the number of sites is limited, as more sites are occupied by IRF from the sample, fewer sites are left to bind IRF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IRF concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Rheumatoid Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between RF and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Rheumatoid Factor ELISA kit

Summary of the Assay Procedure for Rat Rheumatoid Factor ELISA kit

Citations of Rat Rheumatoid Factor ELISA kit

E02R0004 has been referenced in the below publications:

祖师麻肠溶制剂的药效学研究。

The effect of trichosanthis on the immune function of adjuvant-induced arthritis rats.


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