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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Runt Related Transcription Factor 2 ELISA kit (E02R0098)

  • Developmental Biology
  • Stem cells
  • Epigenetics and Nuclear Signaling

E02R0098 Rat Runt Related Transcription Factor 2 ELISA kit

The Rat Runt Related Transcription Factor 2 ELISA kit can be used to identify samples from the rat species. Runt Related Transcription Factor 2 can also be called RUNX2, AML3, CBF-alpha-1, CBFA1, CCD, CCD1, CLCD, OSF-2, OSF2, PEA2aA, PEBP2aA, runt related transcription factor 2, Runx2 mRNA, RUNX family transcription factor 2.

Products

Specifications of Rat Runt Related Transcription Factor 2 ELISA kit

Product Information

Cat. No.

E02R0098

Product Name

Rat Runt Related Transcription Factor 2 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

RUNX2 ELISA kit uses an anti-RUNX2 antibody and an RUNX2-HRP conjugate in a competitive enzyme immunoassay method. RUNX2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-RUNX2 antibody binding site between RUNX2 from samples and RUNX2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of RUNX2. Since the number of sites is limited, as more sites are occupied by RUNX2 from the sample, fewer sites are left to bind RUNX2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RUNX2 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Runt Related Transcription Factor 2 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between RUNX2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Runt Related Transcription Factor 2 ELISA kit

Summary of the Assay Procedure for Rat Runt Related Transcription Factor 2 ELISA kit

Citations of Rat Runt Related Transcription Factor 2 ELISA kit

E02R0098 has been referenced in the below publications:

Control of MSC Differentiation by Tuning the Alkyl Chain Length of Phenylboroinc Acid Based Low-molecular-weight Gelators.

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