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BlueGene Biotech Rat soluble Haemoglobin Scavenger Receptor ELISA kit (E02C0210)

E02C0210 Rat soluble Haemoglobin Scavenger Receptor ELISA kit

The Rat soluble Haemoglobin Scavenger Receptor ELISA kit can be used to identify samples from the rat species. soluble Haemoglobin Scavenger Receptor can also be called M130, MM130, SCARI1, CD163 molecule, sCD163.

Products

Specifications of Rat soluble Haemoglobin Scavenger Receptor ELISA kit

Product Information

Cat. No.

E02C0210

Product Name

Rat soluble Haemoglobin Scavenger Receptor ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

sCD163 ELISA kit uses an anti-sCD163 antibody and an sCD163-HRP conjugate in a competitive enzyme immunoassay method. sCD163-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-sCD163 antibody binding site between sCD163 from samples and sCD163-HRP conjugate, the intensity of the color is inversely proportional to the concentration of sCD163. Since the number of sites is limited, as more sites are occupied by sCD163 from the sample, fewer sites are left to bind sCD163-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sCD163 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat soluble Haemoglobin Scavenger Receptor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between sCD163 and analogues was observed.


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Summary of the Assay Procedure for Rat soluble Haemoglobin Scavenger Receptor ELISA kit

Summary of the Assay Procedure for Rat soluble Haemoglobin Scavenger Receptor ELISA kit

Citations of Rat soluble Haemoglobin Scavenger Receptor ELISA kit

E02C0210 has been referenced in the below publications:

Urinary Soluble CD163 in Active Renal Vasculitis.

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