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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Von Willebrand Factor ELISA kit

E02V0001 Rat Von Willebrand Factor ELISA kit

The Rat Von Willebrand Factor ELISA kit can be used to identify samples from the rat species. Von Willebrand Factor can also be called F8VWF, VWD, von Willebrand antigen 2, Vwf.

Specifications of Rat Von Willebrand Factor ELISA kit

Product Information

Cat. No.

E02V0001

Product Name

Rat Von Willebrand Factor ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Vwf ELISA kit uses an anti-Vwf antibody and an Vwf-HRP conjugate in a competitive enzyme immunoassay method. Vwf-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Vwf antibody binding site between Vwf from samples and Vwf-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Vwf. Since the number of sites is limited, as more sites are occupied by Vwf from the sample, fewer sites are left to bind Vwf-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Vwf concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Von Willebrand Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Vwf and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Von Willebrand Factor ELISA kit

Summary of the Assay Procedure for Rat Von Willebrand Factor ELISA kit

Citations of Rat Von Willebrand Factor ELISA kit

E02V0001 has been referenced in the below publications:

Expression of vWF in Kidney of diabetic rats and effeet of danhong.

Influence of Endothelial Function and Blood Rheology in Diabetic Rats by Paeonol and Tanshinol.

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