E02T0040 Rat Thyroxine ELISA kit
The Rat Thyroxine ELISA kit can be used to identify samples from the rat species. Thyroxine can also be called 3,5,3',5'-Tetraiodothyronine, L-Thyroxine, Levothyroxine, T4.
Specifications of Rat Thyroxine ELISA kit
Product Information | |
Cat. No. | E02T0040 |
Product Name | Rat Thyroxine ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ug/mL |
Sensitivity | 0.1 ug/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ug/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ug/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ug/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD F (0.5mL) | 10 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
T4 ELISA kit uses an anti-T4 antibody and an T4-HRP conjugate in a competitive enzyme immunoassay method. T4-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-T4 antibody binding site between T4 from samples and T4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of T4. Since the number of sites is limited, as more sites are occupied by T4 from the sample, fewer sites are left to bind T4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The T4 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Thyroxine ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between T4 and analogues was observed. | |
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Summary of the Assay Procedure for Rat Thyroxine ELISA kit
Citations of Rat Thyroxine ELISA kit
E02T0040 has been referenced in the below publications：
Oral exposure to dibutyl phthalate exacerbates chronic lymphocytic thyroiditis through oxidative stress in female Wistar rats.
The Influence of Gibberellic Acid Exposure on the Pubertal Development of SD Rats.
Exposure to DBP and high iodine aggravates autoimmune thyroid disease through increasing the levels of IL-17 and thyroid-binding globulin in wistar rats.
