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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Thyroxine ELISA kit

E02T0040 Rat Thyroxine ELISA kit

The Rat Thyroxine ELISA kit can be used to identify samples from the rat species. Thyroxine can also be called 3,5,3',5'-Tetraiodothyronine, L-Thyroxine, Levothyroxine, T4.

Specifications of Rat Thyroxine ELISA kit

Product Information

Cat. No.

E02T0040

Product Name

Rat Thyroxine ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ug/mL

Sensitivity

0.1 ug/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

0.5 ug/mL

1 vial

STANDARD C (0.5mL)

1.0 ug/mL

1 vial

STANDARD D (0.5mL)

2.5 ug/mL

1 vial

STANDARD E (0.5mL)

5.0 ug/mL

1 vial

STANDARD F (0.5mL)

10 ug/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

T4 ELISA kit uses an anti-T4 antibody and an T4-HRP conjugate in a competitive enzyme immunoassay method. T4-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-T4 antibody binding site between T4 from samples and T4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of T4. Since the number of sites is limited, as more sites are occupied by T4 from the sample, fewer sites are left to bind T4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The T4 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Thyroxine ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between T4 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Thyroxine ELISA kit

Summary of the Assay Procedure for Rat Thyroxine ELISA kit

Citations of Rat Thyroxine ELISA kit

E02T0040 has been referenced in the below publications:

Oral exposure to dibutyl phthalate exacerbates chronic lymphocytic thyroiditis through oxidative stress in female Wistar rats.

The Influence of Gibberellic Acid Exposure on the Pubertal Development  of SD Rats.

Exposure to DBP and high iodine aggravates autoimmune thyroid disease through increasing the levels of IL-17 and thyroid-binding globulin in wistar rats.


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