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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Troponin I, cardiac muscle ELISA kit

E02T0491 Rat Troponin I, cardiac muscle ELISA kit

The Rat Troponin I, cardiac muscle ELISA kit can be used to identify samples from the rat species. Troponin I, cardiac muscle can also be called TNNI3, CMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI, troponin I3, cardiac type.

Specifications of Rat Troponin I, cardiac muscle ELISA kit

Product Information

Cat. No.

E02T0491

Product Name

Rat Troponin I, cardiac muscle ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/mL

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TNNI3 ELISA kit uses an anti-TNNI3 antibody and an TNNI3-HRP conjugate in a competitive enzyme immunoassay method. TNNI3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TNNI3 antibody binding site between TNNI3 from samples and TNNI3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TNNI3. Since the number of sites is limited, as more sites are occupied by TNNI3 from the sample, fewer sites are left to bind TNNI3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNNI3 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Troponin I, cardiac muscle ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TNNI3 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Troponin I, cardiac muscle ELISA kit

Summary of the Assay Procedure for Rat Troponin I, cardiac muscle ELISA kit

Citations of Rat Troponin I, cardiac muscle ELISA kit

E02T0491 has been referenced in the below publications:

Combination of ketamine and xylazine exacerbates cardiac dysfunction in severely scalded rats during the shock stage.

Protective effect of different doses of anidoamine against myocardial myocardial ischemia/reperfusion injury and mechanism mediated by mitochondrial ATP-sensitive potassium channel.

Protective Effect of Bisoprolol Preconditioning on Myocardial Ischemia Reperfusion Injury in Rats by the Medium of PI3K/AKT/GS3K-b.

Experimental Study On Cardioprotection and Mechanism Of Combined Limb Remote Ischemic Postconditioning and Sevoflurane postcondmoning Against Rat Myocardial Ischemia-Reperfusion Injury and Its mechanism.


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