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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Interleukin 10 ELISA kit

E14I0023 Sheep Interleukin 10 ELISA kit

Sheep Interleukin 10 ELISA kit is suitable for the detection of samples from sheep species. Interleukin 10 can also be called Interleukin-10 precursor, Cytokine synthesis inhibitory factor, CSIF, IL10A, Interleukin10, TGIF, IL 10, IL10.

Specifications of Sheep Interleukin 10 ELISA kit

Product Information

Cat. No.

E14I0023

Product Name

Sheep Interleukin 10 ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL 10 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 10 antibody and an IL 10-HRP conjugate. The assay sample and buffer are incubated together with IL 10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 10 concentration since IL 10 from samples and IL 10-HRP conjugate compete for the anti-IL 10 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 10 from the sample, fewer sites are left to bind IL 10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 10 concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Interleukin 10 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL 10 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Sheep Interleukin 10 ELISA kit

Summary of the Assay Procedure for Sheep Interleukin 10 ELISA kit

Citations of Sheep Interleukin 10 ELISA kit

E14I0023 has been referenced in the below publications:

Assessment Model ofGeneral Disease Resistance With Principal Component Analysis in Sheeps.

The Comparison of Some Blood Immune Parameters in Sheep.

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