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  • host cell dna residue detection kit
  • host cell dna residue detection kit

BlueGene Biotech Vero Host Cell DNA Residue Detection Kit-100T

VE-D0100T Vero Host Cell DNA Residue Detection kit

The Vero Host Cell DNA Residue Detection Kit can be used for Quantitative analysis of DNA residue in recombinant protein expressed products, purified intermediate and finished products from the host cell.

Specifications of Vero Host Cell DNA Residue Detection kit

Introduction

This kit adopts Taqman probe fluorescence qPCR method. The kit has the advantages of high specificity and sensitivity by using specific primers & probes, LOQ can reach 1fg/μL level. The preparation process of DNA Control is completely consistent with that of other host cell DNA national standards, therefore it has high purity and no protein and ion interference to ensure the accuracy of the sample quantitative detection. The kit provides DNA Dilution Buffer, which enables good replicate parallelism in a single experiment and good reproducibility between multiple experiments.


Kit Components

DNA Amplification

Components NO.

Components Name

Cat#/Size VE-D100T(100T)

B1

2XqPCR Mix

1.25mL

B2

Primer&Probe Mix

200μL

B3

DNA Dilution Buffer

4×1.5mL

B4

DNA Control (10ng/μL)

50μL

B5

RNase-Free H2O

1mL

B6

50X ROX Reference Dye(Optional)

0.3ml


Shipping and Storage
1All components are shipped on dry ice.
2The kit should be stored at -20℃ and it is recommended to be used within one year. B2 should be stored protected from light.
3

B2/B3/B4 can be stored at -20℃ for 2 years, while B1/B5/B6 can be stored at -20℃ for 1 year. B1/B5/B6 can also be purchased together as a

separate set.


Quality Control on Vero Host Cell DNA Residue Detection kit

Accuracy (Standard: ≦30%)

Intra Variation% 4.8-12.5 

Inter Variation% 14-17

Recovery%

102-117

Limit of Quantitation1fg/μL

Specificity

-




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PCR Reaction System on Vero Host Cell DNA Residue Detection kit


Components

Volume(μL)             

2XqPCR Mix

12.5

Primer&Probe Mix

2

DNA template (control or sample)

5

Add water

5.5

Total Volume

25


  • Mix solution = (number of reaction wells+4) * (12.5+2+5.5)μL (including the volume lost in the 4 wells).


  • The detection range of the standard curve mentioned above is suitable for most experiments and can be adjusted as needed, such as 30fg/μL-300pg/μL.

Criteria for Results

  • Standard Curve: R ²> 0.99; Amplification Efficiency: 90% ≤ Eff% ≤ 110%; Slope: -3.8~-3.1.


  • The recovery rate of spiked samples=(measured value of spiked samples - measured value of samples)/theoretical value of spiked samples * 100%, with a range of 50% -150%.


  • No Template Control (NTC): In the reaction system, replacing the target template with DNA Dilution Buffer while keeping other components unchanged, and the Ct value obtained should be 'Undetermined' or Ct value≥35.



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