En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Pituitary adenylate cyclase activating polypeptide ELISA kit

E01P0585 Human Pituitary adenylate cyclase activating polypeptide ELISA kit

The Human Pituitary adenylate cyclase activating polypeptide ELISA kit can be used to identify samples from the human species. Pituitary adenylate cyclase activating polypeptide can also be called PACAP, ADCYAP1, PRP-48, Pituitary Adenylate Cyclase Activating Peptide, adenylate cyclase activating polypeptide 1.

Specifications of Human Pituitary adenylate cyclase activating polypeptide ELISA kit

Product Information

Cat. No.

E01P0585

Product Name

Human Pituitary adenylate cyclase activating polypeptide ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PACAP ELISA kit uses an anti-PACAP antibody and a PACAP-HRP conjugate in a competitive enzyme immunoassay method. PACAP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PACAP antibody binding site between PACAP from samples and PACAP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PACAP. Since the number of sites is limited, as more sites are occupied by PACAP from the sample, fewer sites are left to bind PACAP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PACAP concentration in each sample is interpolated from this standard curve.


Quality Control on Human Pituitary adenylate cyclase activating polypeptide ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PACAP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Pituitary adenylate cyclase activating polypeptide ELISA kit

Summary of the Assay Procedure for Human Pituitary adenylate cyclase activating polypeptide ELISA kit

Citations of Human Pituitary adenylate cyclase activating polypeptide ELISA kit

E01P0585 has been referenced in the below publications:

No Change in Interictal PACAP Levels in Peripheral Blood in Women With Chronic Migraine.

Is it useful to determine CGRP, VIP, and PACAP in migraine? A cross-sectional study in chronic migraine patients.

Serum CGRP, VIP, and PACAP usefulness in migraine: a case-control study in chronic migraine patients in real clinical practice.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.