E01D0056 Human Dopamine Receptor D5 ELISA kit
The Human Dopamine Receptor D5 ELISA kit can be used to identify samples from the human species. Dopamine Receptor D5 can also be called D1BR, DRD5, DBDR, DRD1B, DRD1L2, dopamine receptor D5, D(1B) dopamine receptor, D(5) dopamine receptor, D1beta dopamine receptor, Dopamine D5 receptor.
Product Information | |
Cat. No. | E01D0056 |
Product Name | Human Dopamine Receptor D5 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
DRD5 ELISA kit uses an anti-DRD5 antibody and an DRD5-HRP conjugate in a competitive enzyme immunoassay method. DRD5-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-DRD5 antibody binding site between DRD5 from samples and DRD5-HRP conjugate, the intensity of the color is inversely proportional to the concentration of DRD5. Since the number of sites is limited, as more sites are occupied by DRD5 from the sample, fewer sites are left to bind DRD5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DRD5 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between DRD5 and analogues was observed. | |
E01D0056 has been referenced in the below publications：
A study on the role of gene DRD5, MNX1 aberrant methylation in diagnosis of type 2 diabetic macrovascular complications.
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