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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Hydroxylysyl Pyridinoline ELISA kit

E11H0033 Bovine Hydroxylysyl Pyridinoline ELISA kit

The Bovine Hydroxylysyl Pyridinoline ELISA kit can be used to identify samples from the bovine species. Hydroxylysyl Pyridinoline can also be called HP, Pyridinoline, PYD.

Specifications of Bovine Hydroxylysyl Pyridinoline ELISA kit

Product Information

Cat. No.

E11A0028

Product Name

Bovine Hydroxylysyl Pyridinoline ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

10-250 nmol/L

Sensitivity

1.0 nmol/L

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 nmol/L

1 vial

STANDARD B (0.5mL)

10 nmol/L

1 vial

STANDARD C (0.5mL)

25 nmol/L

1 vial

STANDARD D (0.5mL)

50 nmol/L

1 vial

STANDARD E (0.5mL)

100 nmol/L

1 vial

STANDARD F (0.5mL)

250 nmol/L

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HP ELISA kit uses an anti-HP antibody and an HP-HRP conjugate in a competitive enzyme immunoassay method. HP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HP antibody binding site between HP from samples and HP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HP. Since the number of sites is limited, as more sites are occupied by HP from the sample, fewer sites are left to bind HP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HP concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Hydroxylysyl Pyridinoline ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Hydroxylysyl Pyridinoline ELISA kit

Summary of the Assay Procedure for Bovine Hydroxylysyl Pyridinoline ELISA kit

Citations of Bovine Hydroxylysyl Pyridinoline ELISA kit

E11A0028 has been referenced in the below publications:

Effect of Different Feeding Patterns on Serum Biochemical Indices and Hormone Content in Dairy Cows.

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