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BlueGene Biotech Human Interleukin 2 ELISA kit

E01I0308 Human Interleukin 2 ELISA kit

The Human Interleukin 2 ELISA kit can be used to identify samples from the human species. Interleukin 2 can also be called IL2, IL 2, Interleukin-2, IL-2, lymphokine, interleukin 2, T-cell growth factor, Aldesleukin, Lymphokine, POIL2, T Cell Growth Factor, TCGF.

Specifications of Human Interleukin 2 ELISA kit

Product Information

Cat. No.

E01I0308

Product Name

Human Interleukin 2 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL2 ELISA kit uses an anti-IL2 antibody and a IL2-HRP conjugate in a competitive enzyme immunoassay method. IL2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL2 antibody binding site between IL2 from samples and IL2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL2. Since the number of sites is limited, as more sites are occupied by IL2 from the sample, fewer sites are left to bind IL2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL2 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Interleukin 2 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL2 and analogues was observed.


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Summary of the Assay Procedure for Human Interleukin 2 ELISA kit

Summary of the Assay Procedure for Human Interleukin 2 ELISA kit

Citations of Human Interleukin 2 ELISA kit

E01I0308 has been referenced in the below publications:

The detections of IL-2 and CD4+CD28-T lymphocyte in Patients with ST-segment elevation myocardial infarction and atorvastatin pretreatment before primary percutaneous coronary intervention.

Effects of IL-8 on the function of human umbilical vein endothelial cell line(HUVEC).

Ultrasound‑targeted microbubble destruction‑mediated Foxp3 knockdown may suppress the tumor growth of HCC mice by relieving immunosuppressive Tregs function.

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