En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine NLR Pyrin Domain Containing Proteins 3 ELISA kit

E11N0593 Bovine NLR Pyrin Domain Containing Proteins 3 ELISA kit

The Bovine NLR Pyrin Domain Containing Proteins 3 ELISA kit can be used to identify samples from the bovine species. NLR Pyrin Domain Containing Proteins 3 can also be called AVP, AGTAVPRL, AII, AII/AVP, C1orf7, CIAS1, CLR1.1, FCAS, FCU, MWS, NALP3, PYPAF1, Cryopyrin, Cold Induced Autoinflammatory Syndrome 1, Caterpiller-Like Receptor 1.1, NLRP3.

Specifications of Bovine NLR Pyrin Domain Containing Proteins 3 ELISA kit

Product Information

Cat. No.

E11N0593

Product Name

Bovine NLR Pyrin Domain Containing Proteins 3 ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

5-100 ng/mL

Sensitivity

1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NLRP3 ELISA kit uses an anti-NLRP3 antibody and an NLRP3-HRP conjugate in a competitive enzyme immunoassay method. NLRP3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NLRP3 antibody binding site between NLRP3 from samples and NLRP3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NLRP3. Since the number of sites is limited, as more sites are occupied by NLRP3 from the sample, fewer sites are left to bind NLRP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NLRP3 concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine NLR Pyrin Domain Containing Proteins 3 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NLRP3 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine NLR Pyrin Domain Containing Proteins 3 ELISA kit

Summary of the Assay Procedure for Bovine NLR Pyrin Domain Containing Proteins 3 ELISA kit

Citations of Bovine NLR Pyrin Domain Containing Proteins 3 ELISA kit

E11N0593 has been referenced in the below publications:

NLRP3 Inflammasome Plays an Important Role in the Pathogenesis of Collagen-Induced Arthritis.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.