E02C1337 Rat calcium/calmodulin-dependent protein kinase-Ⅱ ELISA Kit
The Rat calcium/calmodulin-dependent protein kinase-Ⅱ ELISA Kit can be used to identify samples from the rat species. calcium/calmodulin-dependent protein kinase-Ⅱ can also be called CaM kinase II, CaMKII, CAMK 2.
Product Information | |
Cat. No. | E02C1337 |
Product Name | Rat calcium/calmodulin-dependent protein kinase-Ⅱ ELISA Kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CAMK 2 ELISA kit uses an anti-CAMK 2 antibody and an CAMK 2-HRP conjugate in a competitive enzyme immunoassay method. CAMK 2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CAMK 2 antibody binding site between CAMK 2 from samples and CAMK 2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CAMK 2. Since the number of sites is limited, as more sites are occupied by CAMK 2 from the sample, fewer sites are left to bind CAMK 2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAMK 2 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CAMK 2 and analogues was observed. | |
E02C1337 has been referenced in the below publications：
Apolipoprotein-J blocks increased cell injury elicited by ox-LDL via inhibiting ROSCaMKII pathway.
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