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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Nuclear factor kB ELISA kit

E02N0011 Rat Nuclear factor kB ELISA kit

The Rat Nuclear factor kB ELISA kit can be used to identify samples from the rat species. Nuclear factor kB can also be called NF-KB1, EBP-1, KBF1, NF-Kappa-B, NFKB-P105, NFKB-P50, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1, Nuclear factor NF-kappa-B p105 subunit, NFkB.

Specifications of Rat Nuclear factor kB ELISA kit

Product Information

Cat. No.

E02N0011

Product Name

Rat Nuclear factor kB ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NFkB ELISA kit uses an anti-INFkB antibody and an INFkB-HRP conjugate in a competitive enzyme immunoassay method. INFkB-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-INFkB antibody binding site between INFkB from samples and INFkB-HRP conjugate, the intensity of the color is inversely proportional to the concentration of INFkB. Since the number of sites is limited, as more sites are occupied by INFkB from the sample, fewer sites are left to bind INFkB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INFkB concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Nuclear factor kB ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NFkB and analogues was observed.


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Summary of the Assay Procedure for Rat Nuclear factor kB ELISA kit

Summary of the Assay Procedure for Rat Nuclear factor kB ELISA kit

Citations of Rat Nuclear factor kB ELISA kit

E02N0011 has been referenced in the below publications:

Relationship between serum nuclear factor-kB and serum inflammatory cytokines levels in heart failure following acute myocardial infarction in rats.

The effect of different intensity exercise on diabetes atherosclers and serum levels of NF-KB、IL-6、IL-10 AND TNF-a.

The Effects of Astraglus Polysaccharides on Oxidative Stress and Inflammatory Responses in Severely Burned Rats.

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