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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Interleukin 4 ELISA kit

E12I0007 Chicken Interleukin 4 ELISA kit

Chicken Interleukin 4 ELISA kit is suitable for the detection of samples from Chicken species. Interleukin 4 can also be called IL4, BSF1, BCGF1 ,B Cell Stimulatory Factor 1, Lymphocyte stimulatory factor 1, B Cell Growth Factor, Binetrakin, Pitrakinra, IL 4.

Specifications of Chicken Interleukin 4 ELISA kit

Product Information

Cat. No.

E12I0007

Product Name

Chicken Interleukin 4 ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL4 antibody and an IL4-HRP conjugate. The assay sample and buffer are incubated together with IL4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL4 concentration since IL4 from samples and IL4-HRP conjugate compete for the anti-IL4 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL4 from the sample, fewer sites are left to bind IL4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL4 concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Interleukin 4 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL4 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Interleukin 4 ELISA kit

Summary of the Assay Procedure for Chicken Interleukin 4 ELISA kit

Citations of Chicken Interleukin 4 ELISA kit

E12I0007 has been referenced in the below publications:

Effects and mechanism ofcompound Chinese herbal medicinal polysaccharide on immunomodulatory in different genotype chickens.

Screening of Immune Function Related Gene MHC B-LβII and A Certain Purity of Traditional Chinese Medicine Compound Polysaccharides on Immune Regulation in Chicken.

EFFECT OF CLINOPTILOLITE ON GUT PROTECTION OF BROILERS AND STUDY OF RELEVANT MECHANISM.

The Effects of Chicken Major Histocompatibility Complex(MHC) B-LB Ⅱ Gene Hin1 Ⅰ Locus Polymorphism on Imm hune Function.


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