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BlueGene Biotech Chicken Tumor Necrosis Factor Alpha ELISA kit

E12T0008 Chicken Tumor Necrosis Factor Alpha ELISA kit

Chicken Tumor Necrosis Factor Alpha ELISA kit is suitable for the detection of samples from Chicken species. Tumor Necrosis Factor Alpha can also be called DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2, TNFAlpha, TNF Alpha.

Specifications of Chicken Tumor Necrosis Factor Alpha ELISA kit

Product Information

Cat. No.

E12T0008

Product Name

Chicken Tumor Necrosis Factor Alpha ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TNFAlpha ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TNFAlpha antibody and an TNFAlpha-HRP conjugate. The assay sample and buffer are incubated together with TNFAlpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TNFAlpha concentration since TNFAlpha from samples and TNFAlpha-HRP conjugate compete for the anti-TNFAlpha antibody binding site. Since the number of sites is limited, as more sites are occupied by TNFAlpha from the sample, fewer sites are left to bind TNFAlpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNFAlpha concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Tumor Necrosis Factor Alpha ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TNFAlpha and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Tumor Necrosis Factor Alpha ELISA kit

Summary of the Assay Procedure for Chicken Tumor Necrosis Factor Alpha ELISA kit

Citations of Chicken Tumor Necrosis Factor Alpha ELISA kit

E12T0008 has been referenced in the below publications:

Screening of Immune Function Related Gene MHC B-LβII and A Certain Purity of Traditional Chinese Medicine Compound Polysaccharides on Immune Regulation in Chicken.

Study on Acertain purity of traditional Chinese medicine compound polysaccharides with different MHC B-LβII genotype of chicken immunomodulatory effects receptor TLR2, TLR4 and Immune Signaling Molecule.

EFFECT OF CLINOPTILOLITE ON GUT PROTECTION OF BROILERS AND STUDY OF RELEVANT MECHANISM.

The effect of DL-, L-isomers and DL-hydroxy analog administered at 2 levels as dietary sources of methionine on the metabolic and antioxidant parameters and growth performance of turkeys.


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