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BlueGene Biotech Chicken Macromolecule Adiponectin ELISA kit (E12M0038)

E12M0038 Chicken Macromolecule Adiponectin ELISA kit

Chicken Macromolecule Adiponectin ELISA kit is suitable for the detection of samples from chicken species. Macromolecule Adiponectin can also be called M APN, MADPN.

Products

Specifications of Chicken Macromolecule Adiponectin ELISA kit

Product Information

Cat. No.

E12M0038

Product Name

Chicken Macromolecule Adiponectin ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ug/mL

Sensitivity

0.1 ug/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

1.0 ug/mL

1 vial

STANDARD C (0.5mL)

2.5 ug/mL

1 vial

STANDARD D (0.5mL)

5.0 ug/mL

1 vial

STANDARD E (0.5mL)

10 ug/mL

1 vial

STANDARD F (0.5mL)

25 ug/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

M APN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-M APN antibody and an M APN-HRP conjugate. The assay sample and buffer are incubated together with M APN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the M APN concentration since M APN from samples and M APN-HRP conjugate compete for the anti-M APN antibody binding site. Since the number of sites is limited, as more sites are occupied by M APN from the sample, fewer sites are left to bind M APN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The M APN concentration in each sample is interpolated from this standard curve. .


Quality Control on Chicken Macromolecule Adiponectin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between M APN  and analogues was observed.


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Summary of the Assay Procedure for Chicken Macromolecule Adiponectin ELISA kit

Summary of the Assay Procedure for Chicken Macromolecule Adiponectin ELISA kit

Citations of Chicken Macromolecule Adiponectin ELISA kit

E12M0038 has been referenced in the below publications:

Expression profile of adiponectin and adiponectin receptors in high-fat diet feeding chickens.

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