E12G0112 Chicken Granulocyte Colony Stimulating Factor ELISA kit
Chicken Granulocyte Colony Stimulating Factor ELISA kit is suitable for the detection of samples from Chicken species. Granulocyte Colony Stimulating Factor can also be called G-CSF, GCSF, CSF3, C17orf33, CSF3OS, colony stimulating factor 3.
Product Information | |
Cat. No. | E12G0112 |
Product Name | Chicken Granulocyte Colony Stimulating Factor ELISA kit |
Species | Chicken |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GCSF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GCSF antibody and an GCSF-HRP conjugate. The assay sample and buffer are incubated together with GCSF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCSF concentration since GCSF from samples and GCSF-HRP conjugate compete for the anti-GCSF antibody binding site. Since the number of sites is limited, as more sites are occupied by GCSF from the sample, fewer sites are left to bind GCSF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCSF concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-106 | |
1:4 | 87-108 | |
1:8 | 85-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GCSF and analogues was observed. | |
E12G0112 has been referenced in the below publications:
Effects of Chinese Herbal Compound Polysaccharides on Immune Functions during the Brood Incubation Period in Different MHC B-Lβ II Genotype of Laying Hens.
Effects of compound Chinese herbal medicin al polysaccharides on Immunomodulatory in different MHC B-LBⅡ Genotype Egg-laying Chicks.
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