E12E0406 Chicken Epidermal growth factor Like Domain Protein, Multiple 7 ELISA kit
Chicken Epidermal growth factor Like Domain Protein, Multiple 7 ELISA kit is suitable for the detection of samples from chicken species. Epidermal growth factor Like Domain Protein, Multiple 7 can also be called EGF like domain 7, EGF like domain containing protein 7, EGF like domain multiple 7, EGFL 7, Epidermal growth factor like domain protein 7, EGFL7.
Product Information | |
Cat. No. | E12E0406 |
Product Name | Chicken Epidermal growth factor Like Domain Protein, Multiple 7 ELISA kit |
Species | Chicken |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
EGFL7 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-EGFL7 antibody and an EGFL7-HRP conjugate. The assay sample and buffer are incubated together with EGFL7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EGFL7 concentration since EGFL7 from samples and EGFL7-HRP conjugate compete for the anti-EGFL7 antibody binding site. Since the number of sites is limited, as more sites are occupied by EGFL7 from the sample, fewer sites are left to bind EGFL7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EGFL7 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-106 | |
1:4 | 87-108 | |
1:8 | 85-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between EGFL7 and analogues was observed. | |
E12E0406 has been referenced in the below publications:
The Effect of the Compound Traditional Chinese Medicine on Endothelium Derived Multiplication Factor in Lung of Broilers with Ascites Syndrome.
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