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  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Vascular Endothelial Growth Factor Receptor 2 ELISA kit (E12V0004)

E12V0004 Chicken Vascular Endothelial Growth Factor Receptor 2 ELISA kit

Chicken Vascular Endothelial Growth Factor Receptor 2 ELISA kit is suitable for the detection of samples from chicken species. Vascular Endothelial Growth Factor Receptor 2 also be called VEGF Receptor 2, Fetal liver kinase 1, FLK-1, FLK1, KDR, Kinase insert domain receptor (a type III receptor tyrosine kinase), Kinase insert domain receptor, KRD1, Ly73, Protein tyrosine kinase receptor FLK1, VEGFR2.

Products

Specifications of Chicken Vascular Endothelial Growth Factor Receptor 2 ELISA kit

Product Information

Cat. No.

E12V0004

Product Name

Chicken Vascular Endothelial Growth Factor Receptor 2 ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

0.5-10ng/ml

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

0.5 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial



Principle of the Assay

VEGFR2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-VEGFR2 antibody and an VEGFR2-HRP conjugate. The assay sample and buffer are incubated together with VEGFR2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VEGFR2 concentration since VEGFR2 from samples and VEGFR2-HRP conjugate compete for the anti-VEGFR2 antibody binding site. Since the number of sites is limited, as more sites are occupied by VEGFR2 from the sample, fewer sites are left to bind VEGFR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VEGFR2 concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Vascular Endothelial Growth Factor Receptor 2 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

88-116.69%

Linearity

Diluent Ratio

Range %

1:2

95-117

1:4

91-107

1:8

89 -104

Specificity/Cross-reactivity

No significant cross-reactivity or interference between VEGFR2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Vascular Endothelial Growth Factor Receptor 2 ELISA kit

Summary of the Assay Procedure for Chicken Vascular Endothelial Growth Factor Receptor 2 ELISA kit

Citations of Chicken Vascular Endothelial Growth Factor Receptor 2 ELISA kit

E12V0004 has been referenced in the below publications:

The Effect of the Compound Traditional Chinese Medicine on Endothelium Derived Multiplication Factor in Lung of Broilers with Ascites Syndrome.

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