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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Interleukin 15 ELISA kit

E12I0357 Chicken Interleukin 15 ELISA kit

Chicken Interleukin 15 ELISA kit is suitable for the detection of samples from Chicken species. Interleukin 15 can also be called IL15, IL-15, interleukin 15, IL 15.

Specifications of Chicken Interleukin 15 ELISA kit

Product Information

Cat. No.

E12I0357

Product Name

Chicken Interleukin 15 ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL 15 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 15 antibody and an IL 15-HRP conjugate. The assay sample and buffer are incubated together with IL 15-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 15 concentration since IL 15 from samples and IL 15-HRP conjugate compete for the anti-IL 15 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 15 from the sample, fewer sites are left to bind IL 15-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 15 concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Interleukin 15 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL 15 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Interleukin 15 ELISA kit

Summary of the Assay Procedure for Chicken Interleukin 15 ELISA kit

Citations of Chicken Interleukin 15 ELISA kit

E12I0357 has been referenced in the below publications:

Effects of compound Chinese herbal medicin al polysaccharides on Immunomodulatory in different MHC B-LBⅡ Genotype Egg-laying Chicks.


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