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  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Cytochrome P450 ELISA kit (E12C0863)

E12C0863 Chicken Cytochrome P450 ELISA kit

Chicken Cytochrome P450 ELISA kit is suitable for the detection of samples from Chicken species. Cytochrome P450 can also be called P450, CYP, CYP450.

Products

Specifications of Chicken Cytochrome P450 ELISA kit

Product Information

Cat. No.

E12C0863

Product Name

Chicken Cytochrome P450 ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CYP450 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CYP450 antibody and an CYP450-HRP conjugate. The assay sample and buffer are incubated together with CYP450-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition between CYP450 from samples and CYP450-HRP conjugate for the anti-CYP450 antibody binding site, the intensity of the color is inversely correlated with the concentration of CYP450. As more sites are taken up by CYP450 from the sample, fewer sites are left that can bind CYP450-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration.


Quality Control on Chicken Cytochrome P450 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CYP450 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Cytochrome P450 ELISA kit

Summary of the Assay Procedure for Chicken Cytochrome P450 ELISA kit

Citations of Chicken Cytochrome P450 ELISA kit

E11C0007 has been referenced in the below publications:

Protective effect of Salvia miltiorrhiza polysaccharides on liver injury in chickens.


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