E12C0863 Chicken Cytochrome P450 ELISA kit
Chicken Cytochrome P450 ELISA kit is suitable for the detection of samples from Chicken species. Cytochrome P450 can also be called P450, CYP, CYP450.
Specifications of Chicken Cytochrome P450 ELISA kit
Product Information | |
Cat. No. | E12C0863 |
Product Name | Chicken Cytochrome P450 ELISA kit |
Species | Chicken |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CYP450 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CYP450 antibody and an CYP450-HRP conjugate. The assay sample and buffer are incubated together with CYP450-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition between CYP450 from samples and CYP450-HRP conjugate for the anti-CYP450 antibody binding site, the intensity of the color is inversely correlated with the concentration of CYP450. As more sites are taken up by CYP450 from the sample, fewer sites are left that can bind CYP450-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. |
Quality Control on Chicken Cytochrome P450 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-106 | |
1:4 | 87-108 | |
1:8 | 85-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CYP450 and analogues was observed. | |
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Summary of the Assay Procedure for Chicken Cytochrome P450 ELISA kit
Citations of Chicken Cytochrome P450 ELISA kit
E11C0007 has been referenced in the below publications:
Protective effect of Salvia miltiorrhiza polysaccharides on liver injury in chickens.
