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E01A0004 Human Antioncogene P21 Protein/Cyclin Dependent Kinase Inhibitor 1 ELISA Kit

Human Antioncogene p21 protein/Cyclin dependent kinase inhibitor 1 ELISA kit is suitable for the detection of samples from human species.

Specifications of E01A0004 Human p21 ELISA Kit

Porduct's Information

Cat.NO.

E01A0004

Porduct's Name

Human Antioncogene p21 protein/Cyclin dependent kinase inhibitor 1 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

p21 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- p21 antibody and an p21 -HRP conjugate. The assay sample and buffer are incubated together with p21 -HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the p21 concentration since p21 from samples and p21 -HRP conjugate compete for the anti- p21 antibody binding site. Since the number of sites is limited, as more sites are occupied by p21 from the sample, fewer sites are left to bind p21 -HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The p21 concentration in each sample is interpolated from this standard curve.


Quality Control On Human P21 ELISA Kit

CV

Intra Variation% <10%

Inter Variation% <12%

Recovery%

92-105

Linearity

Diluent ratio

Range %

1:2

95-103

1:4

91-107

1:8

92-109

Specificity/Cross-reactivity

No significant cross-reactivity or interference between p21 and analogues was observed.


BlueGene Biotech Product Show

Summary of The Assay Procedures For E01A0004 Human p21 ELISA Kit

Summary of The Assay Procedures For E01A0004 Human p21 ELISA Kit

Citations of Human Antioncogene P21 Protein/Cyclin Dependent Kinase Inhibitor 1 ELISA Kit

Serum p16 and p21 protein expression among people living in Bama county of Guangxi

Product: E01A0001 Human Antioncogene p16 protein/Cyclin Dependent Kinase Inhibitor 2A ELISA kit;


Association between Polycyclic Aromatic Hydrocarbons and DNA damage serum levels of P53 and P21 proteins in neonates from an E-waste Recycling Area

Product: E01P0041 Human P53 ELISA kit

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