E01A0004 Human Antioncogene P21 Protein/Cyclin Dependent Kinase Inhibitor 1 ELISA kit
Human Antioncogene p21 protein/Cyclin dependent kinase inhibitor 1 ELISA kit is suitable for the detection of samples from human species. Antioncogene p21 protein/Cyclin dependent kinase inhibitor 1 can also be called CDK-interacting protein 1, Melanoma differentiation-associated protein 6, CAP20, WAF1/CIP1, SDI1, MDA-6, PIC1, CDKN1, p21Cip1/Waf1, p21Waf1/Cip1, p21.
Product Information | |
Cat. No. | E01A0004 |
Product Name | Human Antioncogene p21 protein/Cyclin dependent kinase inhibitor 1 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50ng/mL |
Sensitivity | 0.1ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
p21 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- p21 antibody and an p21 -HRP conjugate. The assay sample and buffer are incubated together with p21 -HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the p21 concentration since p21 from samples and p21 -HRP conjugate compete for the anti- p21 antibody binding site. Since the number of sites is limited, as more sites are occupied by p21 from the sample, fewer sites are left to bind p21 -HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The p21 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 92-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 95-103 | |
1:4 | 91-107 | |
1:8 | 92-109 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between p21 and analogues was observed. | |
E01A0004 has been referenced in the below publications:
Serum p16 and p21 protein expression among people living in Bama county of Guangxi.
Association between Polycyclic Aromatic Hydrocarbons and DNA damage serum levels of P53 and P21 proteins in neonates from an E-waste Recycling Area.
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