Human Anti livin antibody ELISA kit is suitable for the detection of samples from human species. Anti livin antibody can also be called as Anti BIRC7; Anti KIAP; Anti LIVIN; Anti ML-IAP; Anti MLIAP; Anti RNF50; Anti baculoviral IAP repeat containing 7.
Specifications of E01A0023 Human Anti Livin ELISA Kit
Porduct's Information | |
Cat.NO. | E01A0023 |
Porduct's Name | Human Anti livin antibody ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Anti livin ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Anti livin antibody and an Anti livin-HRP conjugate. The assay sample and buffer are incubated together with Anti livin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti livin concentration since Anti livin from samples and Anti livin-HRP conjugate compete for the anti-Anti livin antibody binding site. Since the number of sites is limited, as more sites are occupied by Anti livin from the sample, fewer sites are left to bind Anti livin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti livin concentration in each sample is interpolated from this standard curve. |
Quality Control On Human Anti livin ELISA Kit
CV | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery% | 89-113 | |
Linearity | Diluent ratio | Range % |
1:2 | 96-105 | |
1:4 | 92-107 | |
1:8 | 87-112 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Anti livin and analogues was observed. | |
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Summary of The Assay Procedures For E01A0023 Human Anti livin ELISA Kit
Citations of Human Anti livin Antibody ELISA Kit
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