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E01A0023 Human Anti livin Antibody ELISA Kit

Human Anti livin antibody ELISA kit is suitable for the detection of samples from human species. Anti livin antibody can also be called as Anti  BIRC7; Anti KIAP; Anti LIVIN; Anti ML-IAP; Anti MLIAP; Anti RNF50; Anti baculoviral IAP repeat containing 7.

Specifications of E01A0023 Human Anti Livin ELISA Kit

Porduct's Information

Cat.NO.

E01A0023

Porduct's Name

Human Anti livin antibody ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

0.5-10ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C



Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Anti livin ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Anti livin antibody and an Anti livin-HRP conjugate. The assay sample and buffer are incubated together with Anti livin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti livin concentration since Anti livin from samples and Anti livin-HRP conjugate compete for the anti-Anti livin antibody binding site. Since the number of sites is limited, as more sites are occupied by Anti livin from the sample, fewer sites are left to bind Anti livin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti livin concentration in each sample is interpolated from this standard curve.


Quality Control On Human Anti livin ELISA Kit

CV

Intra Variation% <10%

Inter Variation% <12%

Recovery%

89-113

Linearity

Diluent ratio

Range %

1:2

96-105

1:4

92-107

1:8

87-112

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Anti livin and analogues was observed.


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Summary of The Assay Procedures For E01A0023 Human Anti livin ELISA Kit

Summary of The Assay Procedures For E01A0023 Human Anti livin ELISA Kit

Citations of Human Anti livin Antibody ELISA Kit

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