E02A0012 Rat Anti Spermiogenesis Antibody ELISA kit
Rat Anti spermiogenesis Antibody ELISA kit is suitable for the detection of samples from Rat species. Anti Spermiogenesis Antibody can also be called Anti-sperm autoantibodies, Anti-spermatozoal antibodies, Sperm agglutinating antibodies (SAGA), Sperm immobilizing antibodies (SIA), Sperm-associated antigen (SAA) antibodies.
Product Information | |
Cat. No. | E02A0012 |
Product Name | Rat Anti spermiogenesis Antibody ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ASAb ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ASAb antibody and an ASAb-HRP conjugate. The assay sample and buffer are incubated together with ASAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASAb concentration since ASAb from samples and ASAb-HRP conjugate compete for the anti-ASAb antibody binding site. Since the number of sites is limited, as more sites are occupied by ASAb from the sample, fewer sites are left to bind ASAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASAb concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 94-108% | |
Linearity | Diluent Ratio | Range % |
1:2 | 90-105 | |
1:4 | 91-108 | |
1:8 | 89-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ASAb and analogues was observed. | |
E02A0012 has been referenced in the below publications:
The clinical and Experimental Study on Immunity Infertility with Jiajian Guishengye Combined with acupunture.
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