En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat 3 Hydroxy 3 methylglutaryl CoA reductase ELISA kit

E02H1250 Rat 3 Hydroxy 3 methylglutaryl CoA reductase ELISA kit

The Rat 3 Hydroxy 3 methylglutaryl CoA reductase ELISA kit can be used to identify samples from the rat species. 3 Hydroxy 3 methylglutaryl CoA reductase can also be called 3-hydroxy-3-methylglutaryl CoA; 3-hydroxy-3-methylglutaryl coenzyme A, HMGCR.

Specifications of Rat 3 Hydroxy 3 methylglutaryl CoA reductase ELISA kit

Product Information

Cat. No.

E02H1250

Product Name

Rat 3 Hydroxy 3 methylglutaryl CoA reductase ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml

1 vial

STANDARD B (0.5mL)

1.0 ng/ml

1 vial

STANDARD C (0.5mL)

2.5 ng/ml

1 vial

STANDARD D (0.5mL)

5.0 ng/ml

1 vial

STANDARD E (0.5mL)

10 ng/ml

1 vial

STANDARD F (0.5mL)

25 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HMGCR ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HMGCR. Standards or samples are then added to the microtiter plate wells and HMGCR if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HMGCR present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HMGCR are added to each well to “sandwich” the HMGCR immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HMGCR and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMGCR concentration in each sample is interpolated from this standard curve.


Quality Control on Rat 3 Hydroxy 3 methylglutaryl CoA reductase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HMGCR and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat 3 Hydroxy 3 methylglutaryl CoA reductase ELISA kit

Summary of the Assay Procedure for Rat 3 Hydroxy 3 methylglutaryl CoA reductase ELISA kit

Citations of Rat 3 Hydroxy 3 methylglutaryl CoA reductase ELISA kit

E02H1250 has been referenced in the below publications:

THE STUDY ON REGULATING EFFECTS AND MECHANISM OF LAMINARLA JAPONICA ON SERUM LIPID OF EXPERIMENTAL HYPERLIPENMIA.

何首乌三种有效成分不同配比对高脂血症大鼠胆固醇代谢影响的实验研究。


Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.