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BlueGene Biotech Rat 3 Hydroxy 3 methylglutaryl CoA reductase ELISA kit

E02H1250 Rat 3 Hydroxy 3 methylglutaryl CoA reductase ELISA kit

The Rat 3 Hydroxy 3 methylglutaryl CoA reductase ELISA kit can be used to identify samples from the rat species. 3 Hydroxy 3 methylglutaryl CoA reductase can also be called 3-hydroxy-3-methylglutaryl CoA; 3-hydroxy-3-methylglutaryl coenzyme A, HMGCR.

Specifications of Rat 3 Hydroxy 3 methylglutaryl CoA reductase ELISA kit

Product Information

Cat. No.

E02H1250

Product Name

Rat 3 Hydroxy 3 methylglutaryl CoA reductase ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml

1 vial

STANDARD B (0.5mL)

1.0 ng/ml

1 vial

STANDARD C (0.5mL)

2.5 ng/ml

1 vial

STANDARD D (0.5mL)

5.0 ng/ml

1 vial

STANDARD E (0.5mL)

10 ng/ml

1 vial

STANDARD F (0.5mL)

25 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HMGCR ELISA kit uses an anti-HMGCR antibody and an HMGCR-HRP conjugate in a competitive enzyme immunoassay method. HMGCR-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HMGCR antibody binding site between HMGCR from samples and HMGCR-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HMGCR. Since the number of sites is limited, as more sites are occupied by HMGCR from the sample, fewer sites are left to bind HMGCR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMGCR concentration in each sample is interpolated from this standard curve.


Quality Control on Rat 3 Hydroxy 3 methylglutaryl CoA reductase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HMGCR and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat 3 Hydroxy 3 methylglutaryl CoA reductase ELISA kit

Summary of the Assay Procedure for Rat 3 Hydroxy 3 methylglutaryl CoA reductase ELISA kit

Citations of Rat 3 Hydroxy 3 methylglutaryl CoA reductase ELISA kit

E02H1250 has been referenced in the below publications:

THE STUDY ON REGULATING EFFECTS AND MECHANISM OF LAMINARLA JAPONICA ON SERUM LIPID OF EXPERIMENTAL HYPERLIPENMIA.

何首乌三种有效成分不同配比对高脂血症大鼠胆固醇代谢影响的实验研究。


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